Uv ultra ii translucent paper series#

Structure elucidation of organic compounds By also measuring the absorbance at a specific wavelength, the impurities can be detected.Additional peaks can be observed due to impurities in the sample and it can be compared with that of standard raw material.It is one of the best methods for the determination of impurities in organic molecules.The computer stores all the data generated and produces the spectrum of the desired compound.ДЄpplications of UV Spectroscopy Detection of Impurities.Most of the time amplifier is coupled to a pen recorder which is connected to the computer.Generally, the current generated in the photocells is of very low intensity, the main purpose of the amplifier is to amplify the signals many times so we can get clear and recordable signals.The amplifier is coupled to a small servometer.The alternating current generated in the photocells is transferred to the amplifier.This results in the generation of pulsating or alternating currents in the photocells. The intensity of the radiation from the reference cell is stronger than the beam of the sample cell.One of the photocells receives the beam from the sample cell and the second detector receives the beam from the reference.Generally, two photocells serve the purpose of the detector in UV spectroscopy.

Glass canвЂ™t be used for the cells as it also absorbs light in the UV region. These cells are made of either silica or quartz.Both sample and reference solution is contained in the cells.One of the two divided beams is passed through the sample solution and the second beam is passГ© through the reference solution.The beam selected by the slit is monochromatic and further divided into two beams with the help of another prism.

Uv ultra ii translucent paper series#

The various wavelengths of the light source which are separated by the prism are then selected by the slits such the rotation of the prism results in a series of continuously increasing wavelengths to pass through the slits for recording purposes.

The radiation emitted from the primary source is dispersed with the help of rotating prisms.

Most of the spectrophotometers areВ double beam spectrophotometers.

Monochromators generally are composed of prisms and slits.

Tungsten filament lamps are rich in red radiations more specifically they emit the radiations of 375 nm, while the intensity of Hydrogen-Deuterium lamps falls below 375 nm.

Tungsten filament lamps and Hydrogen-Deuterium lamps are the most widely used and suitable light sources as they cover the whole UV region.

Instrumentation or Parts of UV Spectroscopy Light Source